EuroSpine - Spineweek 2008, May 26-31, Nine (9) Abstracts with Links -> pdfs (ISSLS)

[Slackwater]

SpineWeek, main web site


2008 EuroSpine Abstracts Link



"Motion Preservation Treatment"



C1 CERVICAL DISC REPLACEMENT IN PATIENTS WITH AND WITHOUT PREVIOUS ADJACENT LEVEL ACDF SURGERY
Author: FM Phillips, co authors: JJ Regan, A Cappuccino, JG DeVine, JE Ahrens, PC McAfee
Twelve (12) pg pdf 154 KB, PCM and PCM-V implants (Cervitech) five sites of US IDE Study


P177 THE EFFECT OF PLACEMENT OF THE LP PRESTIGE DISC ON THE SEGMENTAL MOTION
Author: M Hernandez, co authors: A Kasis, K Lingutla, T Friesem
Two (2) pg pdf


P179 RADIOGRAPHIC RESULTS OF NUCLEUS REPLACEMENT USING THE DASCOR® DISC ARTHROPLASTY SYSTEM:
RESULTS FROM TWO PROSPECTIVE MULTI-CENTER EUROPEAN STUDIES
Author: M Ahrens; co authors: P Donkersloot, F Martens, P Lauweryns, Z Fekete, U Liljenqvist, PP Varga, A Tsantrizos, H Halm, JC Le Huec
Twelve (12) pg pdf 1,561 KB


P184 Prospective, Randomized, Multi-Center FDA IDE Study of
CHARITÉ Artificial Disc vs. Lumbar Fusion:
Index- and Adjacent-Level Range of Motion at 5-Year Follow-Up
Author: LG Jenis ; co authors: RJ Banco, FH Geisler, RT Holt, ME Majd, N Wharton
Seven (7)pg pdf 110 KB


P186 PROSPECTIVE, RANDOMIZED, MULTICENTER FDA IDE STUDY OF CHARITÉ ARTIFICIAL DISC VS. LUMBAR FUSION: EFFECT AT 5-YEAR FOLLOW-UP OF AGE ON CLINICAL OUTCOMES FOLLOWING LUMBAR ARTHROPLASTY
Author: RJ Banco ; co authors: FH Geisler, RD Guyer , BM Hetzell, RT Holt, ME Majd
Eleven (11)pg pdf 97 KB


P187 THE TOTAL FACET ARTHROPLASTY SYSTEM® (TFAS®) IN THE TREATMENT OF SPINAL STENOSIS:
WORLDWIDE EXPERIENCE WITH LONGEST FOLLOW-UP OF 24 MONTHS
Author: S Webb ; co authors: CW Brown, BL Sachs, D Wiles, A Castellvi, C Wingo, M Halperin, G Bajares, A Perez Oliva, R Prejbeanu
One (1)pg pdf 1.9 MB


P188 WEAR EVALUATION OF A PEEK ON PEEK DISC ARTHROPLASTY DEVICE
Author: T Brown ; co authors: Q Bao, T Kilpela
Three (3) pg pdf 349 KB, Pioneer Surgical, compares to Maverick ProDisc and Charite'


SP1 PROSPECTIVE, RANDOMIZED, MULTICENTER FOOD AND DRUG ADMINISTRATION INVESTIGATIONAL DEVICE EXEMPTION STUDY OF LUMBAR TOTAL DISC REPLACEMENT WITH THE
CHARITÉ ARTIFICIAL DISC VERSUS LUMBAR FUSION,
5 YEAR FOLLOW-UP.
Author: RD Guyer ; co authors: PC McAfee, RJ Banco, F Bitan, A Cappuccino, FH Geisler, H Hochschuler, LG Jenis, JJ Regan, SL Blumenthal
Nine (9) page pdf 124 KB


SP3 HYBRID CONSTRUCT:
ALIF L5S1 AND DISC ARTHROPLASTY L4L5 FOR TWO LEVELS DEGENERATIVE DISC DISEASE
CLINICAL OUTCOMES AT 3 YEARS
Author: S Aunoble ; co authors: JC Le huec, R Meyrat
Twelve (12) pg pdf 518 KB


SP4 PROBABILISTIC FINITE ELEMENT ANALYSIS OF THE LUMBAR SPINE AFTER TOTAL DISC REPLACEMENT
Author: A Rohlmann ; co authors: A Mann, T Zander, G Bergmann
Julius Wolff Institut, Charité-Universitätsmedizin Berlin, Germany
Twelve (12) pg pdf 415 KB

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Slackwater

EuroSpine - SpineWeek did not include all abstracts, presentations on the web site.

2008 ISSLS (International Society for the Study of the Lumbar Spine) meeting held concurrently
has 132 abstracts on this Link

2008 ISSLS has some of the missing EuroSpine / Spineweek abstracts.

2008 ISSLS abstracts are:
© 2008, Lippincott Williams & Wilkins


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



C2 CLINICAL OUTCOME AND RADIOLOGICAL ANALYSIS FOLLOWING TCDR WITH PRODISC C AT THE 24 MONTHS FOLLOW UP
C. Mehren, C.J. Siepe, F. Mackel, F. Grochulla, A. Korge, H.M. Mayer
Orthopädische Klinik München Harlaching - Spine Center, Munich, Germany

C3 5-YEAR RESULTS OF THE PROSPECTIVE, RANDOMIZED, MULTICENTER FDA INVESTIGATIONAL DEVICE EXEMPTION (IDE)
PRODISC®-L TOTAL DISC REPLACEMENT (TDR) CLINICAL TRIAL
R.B. Delamarter, J. Zigler, J. Goldstein
The Spine Institute at St John's Health Center, Santa Monica, CA, Texas Back Institute,
Plano, TX, USA

C4 RESULTS OF THE PROSPECTIVE, RANDOM, MULTI-CENTER FOOD AND DRUG ADMINISTRATION INVESTIGATIONAL DEVICE EXEMPTION STUDY OF THE PRODISC-C TOTAL DISC REPLACEMENT VERSUS ANTERIOR DISCECTOMY AND FUSION FOR THE TREATMENT OF 1-LEVEL SYMPTOMATIC
CERVICAL DISC DISEASE
B.V. Darden, D.B. Murrey, J.E. Zigler, M. Janssen, R.B. Delemarter, J. Goldstein, B. Tay
OrthoCarolina Spine Center, Texas Back Institute, Spine Education Research Institute,
The Spine Institute at St. John, NYU- Hospital for Joint Diseases, USA

C5 COMPARISON OF TOTAL DISC REPLACEMENT WITH LUMBAR FUSION SURGERY A RANDOMISED,CONTROLLED TRIAL WITH TWO-YEAR FOLLOW-UP
S. Berg, H. Tropp
Stockholm Spine Center and University Hospital, Linköping, Sweden

C6 PROSPECTIVE, RANDOMIZED, MULTICENTER FDA IDE STUDY OF CHARITÉ ARTIFICIAL DISC VS. LUMBAR FUSION:
EFFECT AT 5-YEAR FOLLOW-UP OF PRIOR SURGERY ON CLINICAL OUTCOMES FOLLOWING LUMBAR ARTHROPLASTY
P.C. McAfee, R.J. Banco, S.L. Blumenthal, F.H. Geisler, R.D. Guyer, R.T. Holt, M.E. Majd
Scoliosis and Spine Center, Townson, USA

C7 TWO-LEVEL PRODISC®-L INVESTIGATIONAL DEVICE EXEMPTION (IDE) CLINICAL TRIAL RESULTS
vs. ONE-LEVEL PRODISC®-L IDE CLINICAL TRIAL RESULTS
J. Goldstein, R.B. Delamarter, J. Zigler, R.A. Balderston, J.M. Spivak
Institute for Evaluative Research in Orthopedic Surgery, University of Bern, Switzerland

C8 SWISS SPINE: A GOVERNMENTALLY MANDATED HTA-REGISTRY FOR TOTAL DISC ARTHROPLASTY.
RESULTS OF CERVICAL DISC PROSTHESES
E. Schluessmann, L.P. Staub, E. Agayev, P. Moulin, M. Aebi, C. Röder
Swiss Paraplegic Center Notwil, Switzerland, Spine Service Inselspital Bern,
University Hospital, University of Bern, Switzerland

C10 CLINICAL VALIDATION OF SPINE WEAR SIMULATORS WITH LONG-TERM TDR WEAR RATES FOR A MOBILE BEARING POLYETHYLENE TDR
S.M. Kurtz, A. Ianuzzi, R. Siskey, D. MacDonald, A. Cohen, A. Dooris, H. Serhan
Exponent, Inc., Drexel Universit DePuy Spine, Philadelphia, USA

C47 COMPARISON OF MAGNETIC RESONANCE IMAGING AND COMPUTED TOMOGRAPHY IN PREDICTING FACET ARTHROSIS IN THE CERVICAL SPINE
Ronald Lehman, Melvin Helgeson, Kathryn Keeler, Torphong Bunmaprasert, K. Daniel Riew
Walter Reed Army Medical Center, Orthopaedics and Rehabilitation, Washington, DC,
United States

C68 HETEROTOPIC OSSIFICATION (HO) AT THE INDEX LEVEL AFTER PRODISC®-C CERVICAL TOTAL DISC REPLACEMENT (TDR) SURGERY: WHAT IS THE CLINICAL SIGNIFICANCE?
Michael Janssen, Jeff Goldstein, Daniel Murrey, Rick Delamarter
Center for Spinal Disorders, Thornton, United States

C73 A PROSPECTIVE RANDOMIZED COMPARISON OF CERVICAL TOTAL DISC REPLACEMENT TO ANTERIOR CERVICAL FUSION
Richard Guyer, Carl Lauryssen, Scott Blementhal
Texas Back Institute, Plano, United States

C74 CERVICORE DISC REPLACEMENT VS FUSION FOR CERVICAL NERVE ROOT COMPRESSION:
FUNCTIONAL AND OCCUPATIONAL OUTCOMES FROM A PROSPECTIVE, RANDOMIZED MULTICENTER TRIAL
Jean-Jacques Abitbol, Nevan G. Baldwin,, Jim A. Youssef,, Neill M. Wright
California Spine Group, San Diego, United States


C76 ADJACENT LEVEL DEGENERATION AFTER CERVICAL SPINE FUSION: MYTH OR FACT?. AN ATTEMPT TO APPROACH THE TRUTH THROUGH A CONTROLLED STUDY
Carlos Villas, Pablo Diaz De Rada, Matias Alfonso, Ricardo Schlotterbeck
Clinica Universitaria De Navarra, Cirugía Ortopedica Y Traumatología, Pamplona, Spain

C77 ONE AND TWO YEAR CLINICAL RESULTS OF SECURE-C CERVICAL DISC ARTHROPLASTY: A RANDOMIZED PROSPECTIVE CLINICAL TRIAL
J. McConnell, J. M. Marzluff, C. Tomaras, I. Volcan, P. Asdourian, L. Hellbusch
Orthopedic Associates of Allentown, Allentown, PA, United States

AB62 SPORT SPINAL STENOSIS VERSUS DEGENERATIVE SPONDYLOLISTHESIS: COMPARISON OF BASELINE CHARACTERISTICS AND OUTCOMES
A. Pearson, E. Blood, J. Lurie, T. Tosteson, J. Weinstein
Dartmouth Hitchcock Medical Center, Orthopaedics, Lebanon, NH, United States


INTEGRATING THEORETICAL AND EXPERIMENTAL METHODS FOR FUNCTIONAL TISSUE ENGINEERING OF THE ANNULUS FIBROSUS
N. Nerurkar, R. Mauck, D. Elliott,
Philadelphia, PA, USA

A MICROSTRUCTURAL INVESTIGATION OF INTERLAMELLAR CONNECTIVITY AND MECHANICAL DISRUPTION WITHIN THE LUMBAR INTERVERTEBRAL DISC ANNULUS
M. L. Schollum, S. P. Veres, P. A. Robertson, N. D. Broom
Department of Chemical and Materials Engineering, University of Auckland, Auckland, N. Z.
Department of Orthopaedic Surgery, Auckland Hospital, Auckland, New Zealand


AB79 ANALYSIS OF REIMBURSEMENT DECISIONS: THE EXAMPLE OF LUMBAR DISC ARTHROPLASTY
D. Wong, A. Kumar, S. Jatana, G. Ghiselli, S. Bainbridge, D. Pappas, K. Wong
Denver Spine, Greenwood Village, Colorado, United States


AB80 COST-UTILITY ANALYSIS OF PROLONGED CONSERVATIVE CARE VERSUS EARLY SURGERY IN PATIENTS WITH SCIATICA CAUSED BY LUMBAR DISC HERNIATION
W. C. Peul, W. van den Hout, B. Koes
Leiden Universit

AB81 EXPENDITURES AND HEALTH STATUS AMONG ADULTS WITH SPINE PROBLEMS: THE MEDICAL EXPENDITURE PANEL SURVEY
S. Mirza, B. Martin, R. Deyo, J. Turner, B. Comstock, S. Sullivan, W. Hollingworth
University of Washington, Orthopaedics, Seattle, WA, United States

AB85 RISK FACTORS FOR LUMBAR DISC DEGENERATION: AN MRI ANALYSIS OF 130 HEALTHY VOLUNTEERS
M. Kanayama, D. Togawa, T. Terai, T. Hashimoto, K. Shigenobu, F. Oha, K. Nagahama
Hakodate Central General Hospital, Spine Center, Hakodate, Japan


AB92 IS THE ANULAR TEAR STIMULATED DURING LUMBAR PROVOCATIVE DISCOGRAPHY THE SOURCE OF LUMBAR PAIN DURING FUNCTIONAL ACTIVITIES?
M. DePalma, L. Peterson, W. Carne, D. Cifu
Virginia Commonwealth University Spine Center, Physical Medicine and Rehabilitation,
Richmond, United States


C90 LUMBAR ADJACENT SEGMENT DEGENERATION AND DISEASE AFTER ARTHRODESIS AND TOTAL DISK ARTHROPLASTY
James Harrop, Jim Youssef, Mitchell Maltenfort, Peggy Vorwald, Pascal Jabbour,
Christopher Bono, Alex Vaccaro
Thomas Jefferson University, Neurological Surgery, Philadelphia, Pennsylvania, United States


C92 A PROSPECTIVE RANDOMIZED COMPARISON OF TWO LUMBAR TOTAL DISC REPLACEMENTS
Richard Guyer, Andrew Cappuccino, Scott Blumenthal
Texas Back Institute, Plano, United States

C93 F.D.A. I.D.E. PROSPECTIVE RANDOMIZED COMPARISON OF THREE LUMBAR ARTIFICIAL DISC REPLACEMENTS (A.D.R.)
Kenneth Pettine, E. Jeffrey Donner
Rocky Mountain Spine Arthroplasty Specialists, Loveland, United States

C94 WHY LUMBAR ARTIFICIAL DISC REPLACEMENTS (A.D.R.) FAIL
Kenneth Pettine, E. Jeffrey Donner
Rocky Mountain Arthroplasty Specialists, Loveland, United States

C95 RESULTS OF THE PROSPECTIVE, RANDOMIZED, MULTI-CENTER FOOD AND DRUG ADMINISTRATION (FDA) INVESTIGATIONAL DEVICE EXEMPTION (IDE) STUDY OF THE PRODISC®-L TOTAL DISC REPLACEMENT (TDR) VS. CIRCUMFERENTIAL
Jeff Goldstein, Richard Balderston, Jeffrey Spivak, Raymond Linovitz, Jim Zucherman, Frank Cammisa, Harry Herkowitz
New York University/Hospital for Joint Diseases, New York, United States


C97 INTERIM EUROPEAN CLINICAL RESULTS OF AN IN SITU CURED, BALLOON CONTAINED, POLYURETHANE NUCLEUS REPLACEMENT DEVICE
Michael Ahrens, Frédéric Martens, Peter Donkersloot, Zolst Fekete, Philippe Lauweryns,
Henry Halm, Jean Charles Le Huec
University of Luebeck, Clinic for Spine Surgery & Scoliosis Center, Neustadt, Germany


C115 DIFFUSION IN HUMAN LUMBAR DISCS CAN BE ENHANCED PHARMACOLOGICALLY WITH ORAL NIMODIPINE
S. Rajasekaran, J. Naresh Babu, K. S. Murugan
Ganga Hospital, Orthopaedic & Spine Surgery, Coimbatore, India


C118 ANTIADHESION BARRIER GEL IMPROVES CLINICAL OUTCOME IN LUMBAR DISCETOMY: A DOUBLE BLINDED PROSPECTIVE RANDOMISED TRIAL OF OXIPLEX BARRIER GEL
Anand Agarwal, Alan Hammer
Medway Maritime Hospital Universuty of London Associated Hospital, Spinal Unit
Orthopaedic Department, Kingston upon Thames, United Kingdom


AB100 CULTURED HUMAN INTERVERTEBRAL DISC CELLS FROM DISCOGENIC LOW BACK PAIN PATIENTS PROMOTE AXONAL OUTGROWTH OF SENSORY NERVE FIBERS IN VITRO
K. Yamauchi, S. Ohtori, T. Koshi, M. Yamashita, M. Suzuki, S. Orita, K. Takahashi
Graduate School of Medicine, Chiba University, Orthopaedic Surgery, Chiba, Japan
[http://abstracts.spinejournal.com/pt...0035.pdf]ISSLS Link[/url]


AB101 ADIPOSE-DERIVED REGENERATIVE CELL TRANSPLANTATION:
EVALUATING INTERVERTEBRAL DISC REPAIR IN A CANINE MODEL
H.J. Meisel, T. Ganey, W. Hutton, R. Schreiber, M.H. Hedrick
Berufsgenossenschaftliche Kliniken Bergmannstrost, Department Of Neurosurgery, Halle/Saale, Germany
ISSLS Link


AB102 STRUCTURED CO-CULTURE OF STEM CELLS AND DISC CELLS ENHANCES MATRIX SYNTHESIS
A. Apple, Z. Buser, A. J. Kim, R. Schneider, J. Lotz
University of California San Francisco, Orthopaedic Surgery, San Francisco, United States
ISSLS Link


AB103 RNA INTERFERENCE IN NUCLEUS PULPOSUS IN VIVO MEDIATED BY ULTRASOUND GENE THERAPY TECHNIQUE
T. Suzuki, K. Nishida, K. Kakutani, K. Maeno, T. Shimomura, M. Doita, M. Kurosaka
Kobe University Graduate School of Medicine, Orthopaedic Surgery, Kobe, Japan
ISSLS Link


AB106 BMP-2 TGF BETA 1 AND AGE ARE REGULATORS OF CARTILAGE INTERMEDIATE LAYER PROTEIN (CILP) GENE EXPRESSION IN THE INTERVERTEBRAL DISC
T. Yoon, Z. Wang, W. Hutton
Altanta, GA, USA
ISSLS Link
Results
Real-time-PCR demonstrated that CILP mRNA levels were dramatically increased in cells treated with BMP-2 and TGF-ß1 relative to control, with BMP-2 being more potent. BMP-2 and TGF-ß1 induced significantly increased CILP promoter activity, with BMP-2 being more potent. The CILP promoter showed higher activity in old lumbar rabbit disc cells than in young rabbit disc cells. BMP-2 and TGF-ß1 induced substantially higher CILP promoter activity in the old rabbit disc cells than in the young rabbit disc cells.

Consistent with our real-time PCR analysis, BMP-2 induced much higher level of CILP promoter activity than TGF-1 in the old and young rabbits disc AF and NP cells. Taken together, these results demonstrated that the growth factors induced substantially higher CILP promoter activity in the old rabbit disc cells than in the young rabbit disc cells.

Conclusions
BMP-2 is a more potent regulator of CILP expression than TGF-ß and increasing ages also upregulates CLIP. By understanding CILP regulation better, we hope to have better insight into why CILP SNP is a risk factor of lumbar discectomy.



AB107 LOADING AFFECTS OXYGEN DIFFUSIVITY AND HENCE NUTRIENT TRANSPORT INTO THE INTERVERTERAL DISC
T. Grünhagen, P. Winlove, J. Urban
Oxford University, Department of Physiology, Anatomy & Genetics, Oxford, United Kingdom
ISSLS Link


C130 DISC REPLACEMENT ADJACENT TO PREVIOUS CERVICAL FUSION: A BIOMECHANICAL COMPARISON OF HYBRID CONSTRUCT VS. TWO-LEVEL FUSION
F. Phillips, M. Lee, M. Dumonski, L.I. Voronov, S.M. Renner, G. Carandang, R.M. Havey,
A.G. Patwardhan
Musculoskeletal Biomechanics Laboratory, Edward Hines Jr. VA Hospital, Hines, IL, USA


C131 DISCOVER ARTIFICIAL CERVICAL DISC SHELL ANGLE: IMPACT ON FUNCTIONAL SPINAL UNIT ANGLE AND RANGE OF MOTION - POST-OPERATIVE REVIEW OF 75 SUBJECTS
M.J. Krinock, H. Hess, J. Yue, R.G. Viere
Borgess Medical Center, Kalamazoo, USA


C136 HETEROTOPIC OSSIFICATION AT THE INDEX LEVEL AFTER PRODISC®-C SURGERY: WHAT IS THE CLINICAL RELEVANCE?
R. Bertagnoli
Pro Spine, Straubing, Germany


C137 DEMOGRAPHICS, UTILIZATION, AND OUTCOMES WITH ANTERIOR CERVICAL FUSION PROCEDURES:
UNITED STATES, 1990-2004
F.P. Girardi, A.A. Sama, L.K. Gaber, M.C. Besculides, M.K. Urban, F.P. Cammisa,
S.G. Memtsoudis
Hospital for Special Surgery, New York, USA


C138 C3-7 LAMINOPLASTY AND C4-6 LAMINOPLASTY FOR CERVICAL SPONDYLOTIC MYELOPATHY: SAGITTAL ALIGNMENT, RANGE OF MOTION, POSTOPERATIVE UPPER EXTREMITY PALSY, AND SURGICAL OUTCOME
Y. Iizuka, H. Iizuka, T. Nakajima, S. Tsutsumi, Y. Sorimachi, T. Ara, M. Nishinome, T. Seki, K. Takagishi
Department of Orthopaedic Surgery, Gunma University Graduate School of Medicine, Maebashi, Japan



CELL BIOLOGY - Chairs: C. BUNGER and J. URBAN

AB112 EFFECTS OF PEROXYNITRATE ON INTERVERTEBRAL DISC CELLS
K. Wuertz, L. Poveda, M. Hottiger, N. Boos
University of Zurich, Institute of Veterinary Biochemistry and Molecular Biology, Zurich, Switzerland


AB113 MATRIX METALLOPROTEINASE INHIBITOR PROTECTS FROM NEUROINFLAMMATION AND PAIN
H. Kobayashi, S. Chattopadhayay, J. Dolkas, S. Kikuchi, R. Myers, V. Shubayev
Fukushima Medical University, Department of Orthopaedic Surgery, Fukushima, Japan


AB114 IS KERATIN-9 A RELIABLE PHENOTYPE MARKER FOR HUMAN NUCLEUS PULPOSUS CELLS?
S. Grad, M. Alini
AO Research Institute, Biomaterials and Tissue Engineering Program, Davos, Switzerland


AB115 TRANSFORMING GROWTH FACTORS-‚3 STIMULATES PROTEOGLYCAN AND COLLAGEN SYNTHESIS UNDER LOW OXYGEN TENSION BY BOVINE INTERVERTEBRAL DISC CELLS IN ALGINATE BEADS
Y. Abe, K. Asanuma, H.Tonomura, T. Kimura, K. Masuda
Rush Medical College, Orthopedic Surgery, Chicago, United States
ISSLS Link
DISCUSSION
TGF-aˆ3 was most effective in stimulating PG and collagen synthesis under 5% O2, which is similar to the in vivo condition. These results, and the effect of TGF-aˆ3 on mesenchymal stem cells, suggest that an in vivo experiment to reveal the effects of an intradiscal injection of TGF-aˆ3 would be worthwhile."


AB116 GENOME-WIDE ASSOCIATION STUDY OF DEGENERATIVE DISC DISEASE (DDD)
Y-P. Kao, D. Chan, K. Cheung, D. Ho, J. Karppinem, J. Leong, K. Lik, S-P. Yip, K. Cheah,
Y-Q. Song, P.C. Sham
University of Hong Kong, Biochemistry, Hong Kong, Hong Kong
[ulr=http://abstracts.spinejournal.com/pt/re/spineabs/pdfhandler.00152232-200808001-00053.pdf]ISSLS Link[/url]



AB120 THE EFFECT OF PULSED ELECTROMAGNETIC FIELD (PEMF) AND BMP-2 ON INTERVERTEBRAL DISC CELLS
J.H. Kim, T. Yoon, Y. Zhu, Z. Wang, T. Hamasaki, B. J. Simon, W. C. Hutton
Emory University, School of Medicine. Atlanta VA medical Center, Emory Orthopedics and
Spine Center, Atlanta, United States
ISSLS Link
When the cells reached 80% confluence, rhBMP-2 was added (0, 20 ng/ml, and 40 ng/ml). The cells were incubated for 3 days either with or without PEMF. PEMF was applied via identical Helmholtz coils (Biomet, Parsippany, NJ) specially configured to two matched incubators. The expression levels of mRNA for aggrecan, collagen type I and II were measured. Sulfated glycosaminoglycan (sGAG) content in the culture media was also measured (DMMB).

Results:
The results obtained for the human and rabbit cells were very similar. PEMF treatment without rhBMP-2 resulted in nearly doubling of aggrecan and collagen type II mRNA levels. The addition of rhBMP-2 into the media increased the aggrecan and collagen type II mRNA. The combination of rhBMP-2 and PEMF had the highest levels of aggrecan and collagen II mRNA.

In contrast the collagen type I mRNA was relatively unchanged with or without PEMF or rhBMP-2. A similar type of upregulation was seen in sGAG levels.

Discussion:
PEMF increases production of chondrocytic genes and enhances the activity of BMP-2. This is the first report of electro-magnetic field effects of disc cells. If further research show efficacy in vivo, this could be a non-invasive method of altering disc cell biology for humans.

[Harrison]

Excellent, with lots to read for all of us. Thank you for posting.